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ستاره غیر فعالستاره غیر فعالستاره غیر فعالستاره غیر فعالستاره غیر فعال

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بررسی اثرات فارماکولوژیک گياهان دارويي و تهیه فرآورده های گیاهی دارویی

زمینه های دیگر فعالیت

- تهیه عصاره های گیاهی و فراکسیون های آنها متناسب با نوع گیاه و مواد موثره ی آن

 

- تهیه فرمولاسیون های داروئی از عصاره ها و مواد گیاهی

 

- بررسی آثار فارماکولوژیک گیاهان دارویی بر سیستم قلب و عروق، جزایر لانگرهانس،

سیستم عصبی، ...

 

- بررسی سمیت عصاره ها و فرآورده های گیاهی در شرایط درون تنی و برون تنی

 

- مطالعات فارماکولوژیک بالینی با همکاری بخش های بیمارستانی و همکاران بالینی

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ستاره غیر فعالستاره غیر فعالستاره غیر فعالستاره غیر فعالستاره غیر فعال
   

كارشناس مركز
 
 


مهندس آزیتا آقائی


کارشناس ارشد پژوهشی و آزمایشگاهی مرکز

 

  

مدرک تحصیلی: کارشناس ارشد مهندسی هسته ای- پرتو پزشکی

 
   

 
Tel: +98 51 38002273
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امتیاز کاربران

ستاره غیر فعالستاره غیر فعالستاره غیر فعالستاره غیر فعالستاره غیر فعال

 

articles

Hydroalcoholic extract of needles of Pinus eldarica enhances pentobarbital-induced sleep: possible involvement of GABAergic system

F Forouzanfar, A Ghorbani, H Rakhshandeh, M Hosseini

Avicenna Journal of Phytomedicine 6 (4), 449-457

2016

The effect of hydro-alcoholic extract of Achillea millefolium on appetite hormone in rats

M Nematy, M Mazidi, A Jafari, S Baghban, H Rakhshandeh, A Norouzy, ...

Avicenna Journal of Phytomedicine, 1-7

2016

Hypnotic Effect of Nepeta glomerulosa on Pentobarbital-Induced Sleep in Mice

A Hosseini, F Forouzanfar, H Rakhshandeh

Jundishapur Journal of Natural Pharmaceutical Products 11 (1)

2016

THE EFFECT OF APIUM GRAVEO LENS ON DOXORUBICICIN-INDUCED TOXICITY IN H9C2 CARDIO MYOBLASTS

D MAHDIAN, A HOSSEINI, H RAKHSHANDEH

JOURNAL OF ZANJAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES 24 (104 ...

2016

Hypnotic Effect of Ocimum basilicum on Pentobarbital-Induced Sleep in Mice

VR Askari, VB Rahimi, A Ghorbani, H Rakhshandeh

Iranian Red Crescent Medical Journal

2016

Safety evaluation of Phytovagex, a pessary formulation of Nigella sativa, on pregnant rats

R Salarinia, H Rakhshandeh, D Oliaee, SG Ghasemi, A Ghorbani

Avicenna Journal of Phytomedicine 6 (1), 117

2016

Capparis spinosa reduces Doxorubicin-induced cardio-toxicity in cardiomyoblast cells

SH Mousavi, A Hosseini, E Bakhtiari, H Rakhshandeh

Avicenna Journal of Phytomedicine

2015

Sleep prolonging impact of fractions of Ocimum basilicum extract in pentobarbital-induced sleep in mice and possible mechanism.

VB Rahimi, VR Askari, A Ghorbani, H Rakhshandeh

Avicenna Journal of Phytomedicine 5

2015

Treatment of cutaneous leishmaniasis using berberis vulgaris: a case report.

G Haghighi, M Maleki, H Rakhshandeh, M Yusofi

Avicenna Journal of Phytomedicine 5

2015

Assessment of clot lytic effect of Carthamus tinctorius (Golrang).

M Yousefi, H Rakhshandeh, E Delshad, F Mahjoub

Avicenna Journal of Phytomedicine 5

2015

Evaluation of the clot-lytic effect of Capparis spinosa l. extracts.

H Rakhshandeh, M Yousefi, F Mahjoub, E Delshad, A Ghaeni, FH Khani

Avicenna Journal of Phytomedicine 5

2015

The effect of hydroalcoholic extract of cinnamon on the symptoms of morphine withdrawal syndrome in mice.

T Molekara, M Nazari, H Rakhshandeh, L Jarahi, F Forouzanfar

Avicenna Journal of Phytomedicine 5

2015

Antitussive Effect of Plantago lanceolata in Guinea Pigs

MH Boskabady, H Rakhshandah, M Afiat, Z Aelami, S Amiri

Iranian Journal of Medical Sciences 31 (3)

2015

The Effect of Oral Capsules of Saffron on Anxiety and Fatigue During the First Stage of Labor

S Ahmadi, S Azhari, H Jafarzadeh, H Rakhshandeh, R Mazlom

SSU_Journals 23 (2), 1915-1926

2015

Effects of hydroalcoholic extract of Coriandrum sativum on oxidative damage in pentylenetetrazole-induced seizures in rats

R Karami, M Hosseini, T Mohammadpour, A Ghorbani, HR Sadeghnia, ...

Iranian journal of neurology 14 (2), 59

2015

Nausea and vomiting in Iranian Traditional Medicine based on Avicenna’s viewpoint

M Nazari, A Taghizadeh, H Orafaei, H Rakhshandeh, MM Bazzaz

Electronic physician 7 (2), 1047

2015

The effect of hydro-alcoholic extract of Artemisia absinthium on appetite in male rats

SB Taraghdari, M Nematy, M Mazidi, M Kamgar, M Soukhtanloo, ...

Avicenna Journal of Phytomedicine 5 (2), 78

2015

EVALUATION OF THE EFFECT OF MAGNOLIA HERBAL MOUTH WASH ON O'LEARY PLAQUE INDEX

R HAGHGOO, F GHORBANI, H RAKHSHANDEH, JS AMEL, BM ZARE, ...

DANESHVAR MEDICINE 22 (118), 61-66

2015

INVESTIGATION OF ANTIFUNGAL ACTIVITY OF METHANOL AND AQUEOUS EXTRACTS OF WALNUT (JUGLANS REGIA) LEAVES AND PEEL AGAINST CANDIDA SPECIES

P ARJI, ALI NASERI, H RAKHSHANDEH, MJ NAJAFZADEH

JOURNAL OF BIRJAND UNIVERSITY OF MEDICAL SCIENCES 22 (2), 115-124

2015

 

 

امتیاز کاربران

ستاره غیر فعالستاره غیر فعالستاره غیر فعالستاره غیر فعالستاره غیر فعال

Toxicity evaluation of hydroalcoholic extract of Ferula gummosa root

Ahmad Ghorbani a, Reza Mohebbati b, Amir Hossein Jafarian c,

Mohammad Mahdi Vahedi d, Seyed Mahmoud Hosseini e, Mohammad Soukhtanloo f, Hamid Reza Sadeghnia a, d, e, **, Azar hosseini a, *

Traditionally, people use harvested Ferula gummosa for medicinal purposes. However, no information about its safety and toxicity is available. In the present study, the toxicological profile of sub-chronic oral administration of hydroalcoholic extract of F. gummosa radix is evaluated in rats. The extract was orally administrated at 100 and 600 mg/kg to male rats for 28 days. After 28 days, clinical signs, mortality, body weights, food and water consumption, organ weights, hematology, serum biochemistry, as well as histopathological and neurobehavioral changes were examined. Also, the sedative effect of this extract was evaluated in mice at the doses of 100, 600, and 800 mg/kg. Its cytotoxicity against human stromavascular cells and human renal epithelial cells were also evaluated. No lethality or adverse toxic signs were seen during the experimental period. There were no significant changes in body and organ weights, hematology, serum biochemistry, and histopathological examination. The extract decreased the rotarod performance, but did not increase pentobarbital-induced hypnosis. Also, F. gummosa extract significantly decreased cell viability at the concentrations of higher than 400 mg/mL. In conclusion, the sub-chronic toxicity study of F. gummosa hydroalcoholic extract demonstrated the extract to be safe for the tested dosage and route of administration.

Cytotoxicity evaluation of extracts and fractions of five marine sponges from the

Persian Gulf and HPLC fingerprint analysis of cytotoxic extracts

Davood Mahdian1, Milad Iranshahy2, Abolfazl Shakeri2, Azar Hoseini3, Hoda Yavari2, Melika Nazemi4,

Mehrdad Iranshahi2*

Objective: To screen the cytotoxic effects of some marine sponges extracts on HeLa and

PC12 cells.

Methods: Five marine sponges including Ircinia echinata (I. echinata), Dysidea avara,

Axinella sinoxea, Haliclona tubifera and Haliclona violacea were collected from the

Persian Gulf (Hengam Island). The cytotoxic effect of these sponges was evaluated by

using MTT assay. The metabolic high performance liquid chromatography fingerprint of

I. echinata was also carried out at two wavelengths (254 and 280 nm).

Results: Among the sponges tested in this study, the extracts of I. echinata and Dysidea

avara possessed the cytotoxic effect on HeLa and PC12 cells. The obtained fractions

from high performance liquid chromatography were evaluated for their cytotoxic properties

against the cell lines. The isolated fractions did not show significant cytotoxic

properties.

Conclusions: I. echinata could be considered as a potential extract for chemotherapy.

Further investigation is needed to determine the accuracy of mechanism.

 

 Effect of saffron on liver metastases in patients suffering from cancers with liver metastases: A randomized, double blind, placebo-controlled clinical trial

Azar Hosseini1, Seyed Hamed Mousavi2, Anis Ghanbari2, Fatemeh Homaee Shandiz3, Hamid Reza Raziee3, Masoud Pezeshki Rad4, Seyed Hadi Mousavi2,5

 

 Objective: Cancer represents the second cause of mortality in the world. Saffron as a medicinal plant is known for its anti-cancer and anti-depressant properties. In this randomized double blind clinical trial, the effects of saffron on response to treatment in patients suffering from liver metastasis were evaluated.

Materials and Methods: Thirteen patients suffering from liver metastases who referred to Ghaem and Imam Reza hospital, Mashhad, Iran were included in this study and then divided into two different groups. Both groups received chemotherapy regimen. Patients in group one were treated with saffron capsule (50 mg, twice daily) during chemotherapy periods whereas patients in group two received placebo. A sum of the longest diameter were calculated and compared for all lesions in IV contrast CT scan before and after the treatment.

Results: from 13 patients included in this study, six patients quit and seven continued until the end. In saffron-treated group, two patients showed partial and complete response (50%) whereas in placebo group, no response was seen. Also, two deaths in placebo and one in saffron group occurred.

Conclusion: This research suggests that saffron might be useful in patients suffering from liver metastasis. However, further investigations with larger sample size are required.

A review on the effects of Allium sativum (Garlic) in metabolic

syndrome

  1. A.      Hosseini1 · H. Hosseinzadeh2

Abstract The metabolic syndrome is a common problem world-wide and includes abdominal obesity, hypertension, dyslipidemia, and hyperglycemia disorders. It leads to insulin resistance and the development of diabetes mellitus or cardiovascular disease. Allium sativum (garlic) has been documented to exhibit anti-diabetic, hypotensive, and hypolipidemic properties. This suggests a potential role of A. sativum in the management of metabolic syndrome; however, more studies should be conducted to evaluate its effectiveness. In this review, we discussed the most relevant articles to find out the role of A. sativum in different components of metabolic syndrome and cardiovascular disease risk factors. Because human reports are rare, further studies are required to establish the clinical value of A. sativum in

metabolic syndrome.

 

 Hypnotic Effect of Nepeta glomerulosa on Pentobarbital-Induced Sleep in Mice

Azar Hosseini,1 Fatemeh Forouzanfar,2,3 and Hassan Rakhshandeh1,3,*

 

 Abstract

Background: Insomnia or sleeplessness is a disorder characterized by a personal incapability to falling or staying asleep for a desirable period of time, therefore disturb in quality of life.

Objectives: The present study was carried out to investigate the effect of hydro-alcoholic extract of Nepeta glomerulosa and its fractions on sleep-prolonging.

Materials and Methods: The extract and its fractions including ethyl acetat, n-butanol and water were injected to mice and sleep duration as well as sleep latency were recorded. Also, toxicity of the extract was determined in both in vivo and in vitro experiments.

Results: The extract increased sleep duration at 50 - 200 mg/Kg. This hypnotic effect was comparable to that of induced by diazepam. N-butanol fraction could increase sleep duration. The sleep latency was decreased by the extract and n-butanol, but not by other fractions. LD50 value for Nepeta glomerulosa was found to be 2.4 g/Kg. It had no toxicity effect on viability of neuronal cells.

Conclusions: Nepeta glomerulosa potentiates sleeping behaviors without any cytotoxicity. The components responsible for this effect are most likely non-polar agents which found in n-butanol fraction.

Protective effect of pomegranate seed oil against

cisplatin-induced nephrotoxicity in rat

Mohammad Taher Boroushaki, Arezoo Rajabian, Mehdi Farzadnia, Azar

Hoseini, Mojdeh Poorlashkari, Amin Taghavi, Karim Dolati & Gholamreza

Bazmandegan

Purpose: Clinical use of cisplatin is limited by its nephrotoxicity. Cisplatin-induced nephrotoxicity is associated with an increase in oxidative stress, leading ultimately to kidney dysfunction. The aim of this study was to investigate the effect of pomegranate seed oil against nephrotoxicity induced by cisplatin in adult rats. Methods: Animals were divided into four

groups. Group I received corn oil (1 mL/kg). Group II received cisplatin (8 mg/kg). Group III and IV received pomegranate seed oil (PSO) 0.4 mL/kg and 0.8 mL/kg one hour before cisplatin injection for 3 days, respectively. Blood samples were collected by cardiac puncture and used for measuring urea and creatinine concentration. Twenty-hour urine samples were collected to measure protein and glucose concentration. The right kidney fixed in formalin for histological examination and the left kidney was homogenized for measurement of malondialdehyde and total sulfhydryl groups. Results: A significant elevation of serum creatinine, urea, urinary glucose, protein concentrations, and non-significant decrease in total thiol content and increase in MDA level in kidney homogenates were observed in cisplatin-treated rats. Also cisplatin reduced animal’s body weight. Mild-to-moderate tubular cell necrosis, hyaline casts, and vascular congestion were observed in group II. PSO pre-treatment significantly decreased urinary protein, glucose, and serum creatinine concentration. PSO also caused a decrease in serum urea, renal MDA, and increase in thiol content, but the level of these parameters were

not significant. Conclusion: The present results suggest that PSO is an effective agent for the prevention of cisplatin-induced renal dysfunction and oxidative damage in rat.

 

 Pancreatic beta cell protection/regeneration with phytotherapy

Azar Hosseini, Reza Shafiee-Nick, Ahmad Ghorbani*

 

 

 Although currently available drugs are useful in controlling early onset complications of diabetes, serious late onset complications appear in a large number of patients. Considering the physiopathology of diabetes, preventing beta cell degeneration and stimulating the endogenous regeneration of islets will be essential approaches for the treatment of insulin-dependent diabetes mellitus. The current review focused on phytochemicals, the antidiabetic effect of which has been proved by pancreatic beta cell protection/regeneration. Among the hundreds of plants that have been investigated for diabetes, a small fraction has shown the regenerative property and was described in this paper. Processes of pancreatic beta cell degeneration and regeneration were described. Also, the proposed mechanisms for the protective/ regenerative effects of such phytochemicals and their potential side effects were discussed.

The Effect of Apium Graveolens on Doxorubicicin-Induced Toxicity in H9c2 cardiomyoblasts

Mahdian D1, Hosseini A2, Rakhshandeh H2

Background and Objective: Doxorubicin is one of the most effective chemotherapeutic agents against solid tumors. Doxorubicin causes cardio-toxicity via production of free radicals and depletion of anti-oxidants. However, the use of anti-oxidants can decrease doxorubicin-induced cardio-toxicity. Apium graveolens(celery) contains anti-oxidant compounds. Hence, this study was an attempt to figure out if it can protect heart cells against oxidative stress of doxorubicin.

Materials and Methods: The cells were incubated with different concentrations of Apium graveolens(celery) extract for 4 hours which continued in the presence of 5µM doxorubicin for 24 hours. Cell viability and the apoptotic induction were determined using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium (MTT) and propidium iodide (PI) staining assays, respectively.

Results: Celery extract increased toxicity of doxorubicin at a concentration of 125-500 µg/ml. Also apoptosis was induced in the presence of doxorubicin at a concentration of 250 and 500µg/ml.

Conclusion: Results showed that celery has no toxicity effect on heart cells alone. However, this extract could not protect cells against doxorubicin- induced cardiotoxicity. A number of mechanisms may increase this effect such as augmentation of free radicals, increased drug delivery into the cells and expression of apoptotic proteins. Further research is needed to decide upon accuracy mechanism.

 

The evaluation of the effects of hydro-alcoholic extract of Brassica oleracea (Red cabbage) on growth inhibition and apoptosis induction in breast cancer cell line MCF-7

Introduction: Brassica vegetables belong to Cruciferous family, and include diverse types of cabbage (green, red, etc.), cauliflower, broccoli, Brussels sprouts and kale. Several studies have shown that consumption of these vegetables can lower incidence rate of cardiovascular and cancer diseases. One of these vegetables is Brassica oleracea. Studies suggested that red cabbage contains beneficial compounds which play a significant role in treatment of different disease such as cancer. This study evaluated cytotoxic and pro-apoptotic effects of hydro-alcoholic extract of Brassica oleracea on cell line MCF-7. Methods: The in-vitro study was carried out on breast cancer cells (MCF-7) at the end of the year 2014 in Pharmacological Research Center of Medicinal Plants. Cells were cultivated in DMEM medium with 10% fetal bovine serum, 100 units/ml penicillin and 100 µg/ml streptomycin. Then, the cells were incubated with different concentrations of extracts. Cell viability was assessed by dint of MTT assay. Apoptotic cells were detected using propidium iodide (PI) staining of DNA fragments by flow cytometry (sub-G1 peak). Results: RC[S1] inhibited the growth of malignant cells in a time-and dose-dependent manner. Following 24 hours, extract undermined cell viability at dose of 2000 µg/ml (P<0.05), after 48 hours increased cell death at doses of 1000 (P<0.05), 1500 (P<0.05) and 2000 (P<0.01). After 72h, rate of cell death increased at doses of 500 (P<0.05), 1000 (P<0.01), 1500 (P<0.01) and 2000 (P<0.001). Also, the extract induced apoptosis at doses of 1000 (P<0.05), 1500 (P<0.01) and 2000 (P<0.001). Further, the extract did not have any cytotoxicity effect on normal cells after 24, 48 and 72 hours. The IC50 was 2685 µg/ml. Conclusion: The results of this study indicated that red cabbage accelerated cell death in MCF-7 cells via apoptosis induction. Therefore, red cabbage can be considered an effective treatment for breast cancer. © 2014, Mashhad University of Medical Sciences. All rights reserved.

Protective effect of Rheum turkestanikum root against doxorubicin-induced toxicity in H9c2 cells

Azar Hosseinia,∗, Arezoo Rajabianb

Doxorubicin is a chemotherapy drug but its clinical using is limited because of its cardiotoxicity. Reac-tive oxygen species play an important role in the pathological process. The aim of this study is to evaluatethe protective effect of Rheum turkestanicum Janisch., Polygonaceae, against doxorubicin-induced apo-ptosis and death in H9c2 cells. The cells were incubated with different concentrations of R. turkestanicumextract and N-acetylcysteine as positive control for 2 h, followed by incubation with 5  _M doxorubicinfor 24 h. Cell viability and apoptotic induction were determined by using MTT and PI assays, respectively.The level of reactive oxygen species and lipid peroxidation was measured by fluorimetric methods. Doxo-rubicin significantly decreased cell viability which was accompanied by an increase in ROS productionand lipid peroxidation. Pretreatment with R. turkestanicum increased the viability of cardiomyocytes andcould decrease lipid peroxidation and reactive oxygen species generation. Also, R. turkestanicum atten-uated apoptotic induction. N-acetylcysteine at 100  _M reduced the levels of reactive oxygen speciesand lipid peroxidation. But, treating H9c2 cells with N-acetylcysteine did little to protect H9c2 cells fromdoxorubicin-induced cell death. R. turkestanicum exerts protective effect against oxidative stress-inducedcardiomyocytes damage. Our findings showed that R. turkestanicum could exert the cardioprotectiveeffects against doxorubicin-induced toxicity partly by anti-apoptotic activity. Also, N-acetylcysteine pre-vented oxidative stress via reduction of reactive oxygen species and lipid peroxidation. N-acetylcysteineinduced less protective effects than R. turkestanicum extract against doxorubicin-induced cytotoxicity.

PROTECTIVE EFFECT OF LACTUCA SERRIOLA ON DOXORUBICIN-INDUCED TOXICITY IN H9C2 CELLS

AZAR HOSSEINI1* and DAVOOD MAHDIAN2

Abstract: The use of doxorubicin (DOX) is limited by its dose-dependency because of its cardiotoxicity. Reactive oxygen species (ROS) play an important role in the pathological process. The aim of this study is to evaluate the protective effect of Lactuca serriola against DOX-induced apoptosis and death in H9C2 cells. The cells were incubated with different concentrations of extract for 4 h which continued in the presence or absence of 5 μM doxorubicin for 24 h. Cell viability, apoptotic induction and the level of apoptotic proteins were determined by using MTT, PI and immunoblotting assays, respectively. The level of lipid peroxidation was measured by fluorimetric method. DOX significantly decreased cell viability which was accompanied by an increase in ROS production and lipid peroxidation. Pretreatment with Lactuca serriola increased the viability of cardiomyocytes and could decrease lipid peroxidation. Also, Lactuca serriola inhibited the reduction of anti-apoptotic Bcl-2 protein and elevation of apoptotic Bax and caspase-3 proteins. In conclusion, Lactuca serriola exerts protective effect against oxidative stress-induced cardiomyocytes damage. Therefore, it has the potential to be used as cardioprotective agent by the patients with cardiovascular diseases.

 

 Ctotoxic and apoptogenic effects of Perovskia abrotanoides flower extract on MCF-7 and HeLa cell lines

Mohamad Ali Geryani1, Davood Mahdian1, Seyed Hadi Mousavi1,2,3*, Azar Hosseini2

 

 Objective: Perovskia abrotanoides Karel, belongs to the family Lamiaceae and grows wild alongside the mountainous roads inarid and cold climate of Northern Iran. The anti-tumor activity of P. abrotanoides root extract has been shown previously. This study was designed to examine in vitro anti-proliferative and pro-apoptotic effects of flower extract of P. abrotanoides on MCF-7 and Hela cell lines.

Materials and Methods: Cells were cultured in DMEM medium with 10% fetal bovine serum, 100 units/ml penicillin and 100 μg/ml streptomycin and incubated with different concentrations of plant extracts. Cell viability was quantified by MTT assay. Apoptotic cells were determined using propidium iodide (PI) staining of DNA fragmentation by flow cytometry (sub-G1 peak).

Results: P. abrotanoides extract inhibited the growth of malignant cells in a time and dose-dependent manner and 1000 μg/ml of extract following 48h of incubation was the most cytotoxic dose against Hela cell in comparison with other doses; however, in MCF-7 cells,1000 and 500 μg/ml PA induced toxicity at all time points but with different features.. Analysis of flowcytometry histogram of treated cells compared with control cells indicated that the cytotoxic effect is partly due toapoptosis induction.

Conclusion: Hydro-alcoholic extract of P. abrotanoides flowers inhibits the growth of MCF-7 and HeLa cell lines, partly via inducing apoptosis. Their inhibitory effect was increased in a time and dose-dependent manner, especially in MCF7 cells. However, further studies are needed to reveal the mechanisms of P. abrotanoides extract-induced cell death.

 

 Protective effect of pomegranate seed oil against H2O2 -induced oxidative stress in cardiomyocytes

Mehdi Bihamta1, Azar Hosseini2, Ahmad Ghorbani2, Mohammad Taher Boroushaki1,2*

 

 Objective: It has been well documented that oxidative stress is involved in the pathogenesis of cardiac diseases. Previous studies have shown that pomegranate seed oil (PSO) has antioxidant properties. This study was designed to investigate probable protective effects of PSO against hydrogen peroxide (H2O2)-induced damage in H9c2 cardiomyocytes.

Materials and Methods: The cells were pretreated 24 hr with PSO 1 hr before exposure to 200 μM H2O2. Cell viability was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium (MTT) assay. The level of reactive oxygen species (ROS) and lipid peroxidation were measured by fluorimetric methods.

Results: H2O2 significantly decreased cell viability which was accompanied by an increase in ROS production and lipid peroxidation and a decline in superoxide dismutase activity. Pretreatment with PSO increased viability of cardiomyocytes and decrease the elevated ROS production and lipid peroxidation. Also, PSO was able to restore superoxide dismutase activity.

Conclusion: PSO has protective effect against oxidative stress-induced damage in cardiomyocytes and can be considered as a natural cardioprotective agent to prevent cardiovascular diseases.

Please cite this paper as:

Bihamta M, Hosseini A, Ghorbani A, Boroushaki MT. Protective effect of pomegranate seed oil against H2O2 - induced oxidative stress in cardiomyocytes.Avicenna J Phytomed, 2016. Epub ahead of print.

 

 Capparis spinosa reduces Doxorubicin-induced cardio-toxicity in cardiomyoblast cells

Seyed Hadi Mousavi1, 2, 3, Azar Hosseini 1*, Elham Bakhtiari2, Hassan Rakhshandeh1,2

 

 Objective: Doxorubicin (DOX) is an effective anticancer drug but its clinical application is limited because it induces apoptosis in cardiomyocytes and leads to permanent degenerative cardiomyopathy and heart failure possibly due to oxidative stress. Recent studies showed that Capparis spinosa (C. spinose) exhibits potent antioxidant activity. So, in this study, we explored the protective effect of hydro-alcoholic extract of C. spinosa against DOX-induced cytotoxicity in H9c2 cells.

Materials and Methods: Cell viability was quantified by MTT assay. Apoptotic cells were determined using flow cytometry (sub-G1 peak) evaluation of DNA fragmentation following PI staining. Cells were cultured with 5 μM DOX for 24 hr to induce cell damage. H9c2 cells were pretreated with different concentrations (6-200 μg/ml) of C. spinosa extract for 4 hr before DOX treatment in all trials.

Results: Pretreatment with 25, 50, 100 and 200 μg/ml of C. spinosa could increase the viability of H9C2 cells to 72.63 ± 2.8% (p< 0.05), 77.37 ± 1.8% (p< 0.05), 83.56 ± 2.6% (p< 0.001) and 90.9 ± 0.5% (p< 0.001) of control, respectively. Also, C. spinosa decreased apoptotic induction significantly, at the doses of 50 μg/ml (p<0.05), 100 μg/ml (p<0.01) and 200 μg/ml (p<0.001)

Conclusion: Our results showed that C. spinosa could exert cardioprotective effects against DOX-induced toxicity that might be mediated via its antioxidant activity.

 

Kelussia odoratissima potentiates cytotoxicity effect of radiation on HeLa cancer cell line

Abstract

Objectives

 Cervical cancer is a malignancy that is the second most common cause of death from cancer in women throughout the world. The aim of this study was to evaluate the cytotoxic activity of Kelussia odoratissima extract associated with radiotherapy in cervical cancer cells (HeLa cell line).

Materials and methods

The different concentration of extract (25-500µg/ml) was tested in HeLa cell lines. Cell cytotoxicity of extract and association extract/radiation (2Gy/min) was assessed by MTT assay. Apoptosis was assessed using flow cytometric analysis.

Result

Kelussia odoratissima decreased cell viability in the HeLa cell line in a concentration and time dependent manner. When compared to the control Kelussia odoratissima induced a sub-G1 peak in the flow cytometry histogram of treated cells, thus indicating that apoptotic cell death is involved in Kelussia odoratissima -induced toxicity. It was also shown to sensitize cells to radiation-induced toxicity.

Conclusions

Pre-treatment of HeLa cells with the extract derived from Kelussia odoratissima followed by irradiation induced a cytotoxic effect.

Artemisia kopetdaghensis potentiates cytotoxicity effect of radiation on HeLa cancer cell line

Abstract

Antineoplastic effects of molecules derived from plants have recently gained increasing attention as an additive to traditional therapies. The aim of this study was to evaluate the cytotoxic activity of Artemisia kopetdaghensis extract associated with radiotherapy in cervical cancer cells (HeLa cell line).

The different concentration of extract (25-250µg/ml) was tested in HeLa cell lines. Cell cytotoxicity of extract and association extract/radiation (2Gy/min) was assessed by MTT assay. Apoptosis was assessed using flow cytometric analysis.

Artemisia kopetdaghensis decreased cell viability in the HeLa cell line in a concentration- and time - dependent manner. When compared to the control Artemisia kopetdaghensis induced a sub-G1 peak in the flow cytometry histogram of treated cells, thus indicating that apoptotic cell death is involved in Artemisia kopetdaghensis -induced toxicity. It was also shown to sensitize cells to radiation-induced toxicity.

Pre-treatment of HeLa cells with the extract derived from Artemisia kopetdaghensis followed by irradiation induced an additive cytotoxic effect.

Study of radiotherapy-induced cytotoxicity and apoptosis of Ferula gummosa in the HeLa cell line

Abstract

Cervical cancer is the second most common type of cancer among women worldwide, for which radiotherapy is a standard treatment. Ferula gummosaBoiss (Apiaceae) is an extremely precious medicinal plant which naturally grows throughout the Mediterranean and Central Asia. The present study examined the cytotoxic effects on HeLa cells from the induction of apoptosis and radiation sensitivity by Ferula gummosa. HeLa cells were cultured in a RPMI1640 medium, incubated ok different concentrations of Ferula gummosa (0-1000 µg/ml), and afterwards exposed to 2 Gy γ-rays. The cytotoxicity effect was determined by the MTT assay. Via floweytometry, apoptotic cells were quantified by a propidium iodide (PI) staining of DNA fragmentation. Ferula gummosa decreased cell viability in the HeLa cell line in a concentration- and time - dependent manner. When compared to the control Ferula gummosa induced a sub-G1 peak in the flow cytometry histogram of treated cells, thus indicating that apoptotic cell death is involved in Ferula gummosa-induced toxicity. It was also shown to sensitize cells to radiation-induced toxicity and apoptosis. The concurrent use of Ferula gummosa and radiation increases radiation sensitivity and cell death. Therefore, Ferula gummosa can be considered as a potential agent and radiosensitizer in the medical treatment of cancer.

Ph.D. Students Theses:

 

cAMP accumulative agents, inhibit cervical cancer (Hela) and breast cancer (MCF-7) cells proliferation via cAMP-dependent and independent pathways

Objective

Breast and cervical cancer is the most common cancer in the world, and also the major cause of death from cancer among women. cAMP can stimulate cell growth in many cell types while inhibiting growth of cells in others. As shown the growth inhibitory effects of selective PDE3 inhibitors are more effective than non-selective inhibitor. In this study the mechanism of selective and non selective phosphodiesterase inhibitors (PDEI) on cell growth of cervical and breast cancers were investigated.

Material & Methods

Cells were incubated with selective, non selective PDE inhibitors, beta adrenergic receptor agonist, direct stimulator of adenylyl cyclase and doxorubicin. For investigation the mechanism of action and synergistic effects of listed drugs, cells were incubated with two or three of these drugs together.  

Cell viability was measured by MTT assay, synergistic effects of these drugs with doxorubicin measured by MTT assay and explain by compusyn software. Apoptotic cells were determined using PI staining and western blotting. For evaluation of free radicals generation using reactive oxygen spices test. Intracellular cAMP was measured by assay kit.

Results

Results showed that the mechanism of inhibitory effect of amrinone as selective PDE3 inhibitors was dissimilar by cilostamide. As shown amrinone probably can reduce cell growth via free radical generation but cilostamide reduce cell growth maybe via calcium pathway. All of these two pathways activate mitochondrial apoptotic pathway. Isopernalin had no effect in apoptosis but increase the generation of free radicals thus decrease cell growth in time dependent manner. IBMX had toxic effect but less potent that selective PDEI. Forskolin had no effect in generation of free radical, apoptosis and cell growth and decrease the potency of selective and non selective PDEI. Therefore all the drugs that investigate in this article, increase intracellular cAMP and can decrease cell growth of cancerous cell via different mechanism that independent cAMP pathway

Background: Over expression of renin-angiotensin system (RAS) and nuclear factor-kappaB (NF-κB) have major role in many cancers. It has been suggested that angiotensin receptor blockers (ARBs) could reduce the proliferation of cancer cells. Role of NF-κB pathway has been documented in cell proliferation. In this study role of angiotensin II and NF-κB pathway in human cervical cancer cell line (HeLa) and breast cancer (MCF-7) proliferation were studied using olmesartan, a novel Ag II antagonist and Bay11-7082, as NF-κB inhibitor.

Methods: HeLa, MCF-7 and HFF (Human foreskin fibroblast) cells cultured in optimum condition.

Malignant and nonmalignant cells were treated with different concentrations of olmesartan (0.175-5 mM) and Bay11-7082 (6-25 μM) for 24, 48 and 72 h. Cells that were treated with combinations of olmesartan (0.7-5 mM) and Bay11-7082 (12-25μM) incubated for 24 h. Cell viability was determined, by MTT assay. Synergistic activity of olmesartan with Bay11-7082 was analyzed with Compusyn software. Apoptotic cells were determined using PI staining of DNA fragmentation. Apoptotic proteins and IκB concentration was determined with western blotting. The role of ROS was determined using DCF-DA by flow cytometry analysis.

Results: Cell viability decreased with olmesartan and Bay11-7082 in HeLa and MCF-7 cells. At concentrations lower than 0.7 mM, olmesartan had no toxic effect on HeLa and MCF-7 cell lines. Olmesartan showed no significant cytotoxic effect on HFF cell line. Combinations of olmesartan with Bay11-7082 decreased cell viability as compared with single agent treatments. Olmesartan had synergistic activity with Bay11-7082 in HeLa cell line. Olmesartan and Bay11-7082 induced a sub-G1 peak in flow cytometry histogram of treated cells, indicated that apoptosis is involved in olmesartan and Bay11-7082-induced toxicity. Kinetic of ROS assay showed increment of ROS generation starting at 2 h which peaked at 4 h after treatment. Pretreatment with rutin, an antioxidant decreased ROS generation which was consistent with improved viability of olmesartan-treated cells. Olmesartan (5 mM) and Bay11-7082 (25 μM) increased the expression of IκB and apoptotic proteins including Bax and Caspase 3 in HeLa and MCF-7 cell line.

Conclusion: Results imply that; a) olmesartan and Bay11-7082 inhibit the growth of HeLa and MCF-7 cells in a concentration- and time-dependent manner. b) ROS increment could be one of the mechanisms involved in olmesartan-induced toxicity in HeLa and MCF-7 cell lines. c) RAS and NF-κB pathway blockade lead to significant cytotoxicity against tumor cell line. d) Olmesartan probably induces cytotoxic activity via inhibition of NF-κB pathway.

  1. Ahmad Ghorbani, Reza Mohebbati, Amir Hossein Jafarian, Mohammad Mahdi Vahedi, Seyed Mahmoud Hosseini, Mohammad Soukhtanloo, Hamid Reza Sadeghnia, Azar hosseini. Toxicity evaluation of hydroalcoholic extract of Ferula gummosa root. Regulatory Toxicology and Pharmacology 77 (2016) 35e41
  2. AZAR HOSSEINI and DAVOOD MAHDIAN. PROTECTIVE EFFECT OF LACTUCA SERRIOLA ON DOXORUBICIN-INDUCED TOXICITY IN H9C2 CELLS. Acta Poloniae Pharmaceutica n Drug Research, Vol. 73 No. 3 pp. 659n666, 2016
  3. Azar Hosseini, Arezoo Rajabian. Protective effect of Rheum turkestanikum root against doxorubicin-induced toxicity in H9c2 cells. Revista Brasileira de Farmacognosia 26 (2016) 347–351
  4. Mahdian D, Hosseini A, Rakhshandeh H. The Effect of Apium Graveolens on Doxorubicicin-Induced Toxicity in H9c2 Cardiomyoblasts. 2015, Zums Journal

 

  1. Azar Hosseini, Fatemeh Forouzanfar, and Hassan Rakhshandeh.Hypnotic Effect of Nepeta glomerulosa on Pentobarbital-Induced Sleep in mice.  Jundishapur J Nat Pharm Prod. 2016 February; 11(1): e25063
  2. Mohamad Ali GeryaniDavood Mahdian ; Seyed Hadi Mousavi Azar Hosseini. Ctotoxic and apoptogenic effects of Perovskia abrotanoides flower extract on MCF-7 and HeLa cell lines. AJP, Vol. 6, No. 4, Jul-Aug 2016
  3. Azar Hosseini, Seyed Hamed Mousavi, Anis Ghanbari, Fatemeh Homaee Shandiz, Hamid Reza Raziee, Masoud Pezeshki Rad, Seyed Hadi Mousavi. Effect of saffron on liver metastases in patients suffering from cancers with liver metastases: A randomized, double blind, placebo-controlled clinical trial.  AJP, Vol. 5, No. 5, Sep-Oct 2015
  4.  Davoud Mahdian, Azar Hosseini, Seyed Hadi Mousavi, Mahdi Bihamta and Mohammad Mahdi Vahedi. The Evaluation of the Effects of Hydro-Alcoholic Extract of Brassica oleracea (Red Cabbage) on Growth Inhibition and Apoptosis Induction in Breast Cancer Cell Line MCF-7. IJOGI, Vol. 18, No. 151, pp. 1-11, Jun 2015
  5. Davood Mahdian, Milad Iranshahy, Abolfazl Shakeri, Azar Hoseini, Hoda Yavari, Melika Nazemi, Mehrdad Iranshahi. Cytotoxicity evaluation of extracts and fractions of five marine sponges from the Persian Gulf and HPLC. Asian Pac J Trop Biomed 2015; 5(11): 896–901
  6. Mohammad Taher Boroushaki, Azar Hosseini, Karim Dolati, Hamid Mollazadeh, Arezoo Rajabian. PROTECTIVE EFFECT OF POMEGRANATE SEED OIL AGAINST MERCURIC CHLORIDE-INDUCED HEPATOTOXICITY IN RAT. Acta Poloniae Pharmaceutica n Drug Research In Press

10.  Azar Hosseini, Reza Shafiee-Nick, Ahmad Ghorbani .Pancreatic beta cell protection/regeneration with phytotherapy. Brazilian journal of Pharmaceutical Science, 2015.

11.  A. Hosseini, H. Hosseinzadeh. A review on the effects of Allium sativum (Garlic) in metabolic syndrome. J Endocrinol Invest 2015.

 

  1.  Mehdi Bihamta, Azar Hosseini, Ahmad Ghorbani, Mohammad Taher Boroushaki. Protective effect of pomegranate seed oil against H2O2 -induced oxidative stress in cardiomyocytes. Avicenna J Phytomed. In Press.

 

  1. Azar Hosseini, Elham Bakhtiari, Seyed Hadi Mousavi. Protective Effect of Hibiscus Sabdariffa in Doxorubicin-induced Cytotoxicity in H9c2 Cardiomyoblast Cells. Iran J Pharm Res, In Press
  2. Azar Hosseini, Elham Bakhtiari, Abolfazl Khajavi Rad, Samira Shahraki, Seyed Hadi Mousavi, Shahrzad Havakhah. The evaluation and comparing of cytotoxic effects of Ferula gummosa gum, Scutellaria lindbergii, Kelussia odoratissima and Artemisia kopetdaghensis extracts on ACHN cell line. Iran J Pharm Res, In Press

 

Davoud Mahdian, Azar Hosseini, Seyed Hadi Mousavi, Mahdi Bihamta and Mohammad Mahdi Vahedi. The Evaluation of the Effects of Hydro-Alcoholic Extract of Brassica oleracea (Red Cabbage) on Growth Inhibition and Apoptosis Induction in Breast Cancer Cell Line MCF-7. 1st International and 22th Iranian Congress of Physiology and Pharmacology. 7-11Sept2015. Kashan University of medical Science (Poster presentation)

Azar Hosseini , Reza Shafiee-Nick , Seyed Hadi Mousavi. Combination of Nigella sativa with Glycyrrhiza glabra and Zingiber officinale augments their protective effects on doxorubicin-induced toxicity in h9c2 cells.The first International Congress of complementary and alternative medicine (Poster presentation)

 

امتیاز کاربران

ستاره غیر فعالستاره غیر فعالستاره غیر فعالستاره غیر فعالستاره غیر فعال

 

 

 

 

 

Articles

 

  1. Ghorbani A, Mohebbati R, Jafarian A, Vahedi MM, Hosseini SM, Soukhtanloo M, Sadeghnia HR, Hosseini A. Toxicity evaluation of hydroalcoholic extract of Ferula gummosa root. Regulatory Toxicology and Pharmacology 2016; 77: 35-41.
  2. Ghorbani A*, Naderi-Meshkinb H. The endocrine regulation of stem cells: physiological importance and pharmacological potentials for cell-based therapy. Current Stem Cell Research & Therapy 2016; 11(1): 19-34.
  3. Tashakori-Sabzevar F, Ramezani M, Hosseinzadeh H, Parizadeh SMR, Movassaghi AR, Ghorbani A, Mohajeri SA. Protective and hypoglycemic effects of celery seed on streptozotocin-induced diabetic rats: experimental and histopathological evaluation. Acta Diabetilogica 2016; in press
  4. Ghorbani A*, Ghazvini K. Using paper presentation breaks during didactic lectures improves learning of physiology in undergraduate students. Advances in Physiology Education; 2016; 40(1): 93-97.
  5. Askari VR, Baradaran Rahimi V, Ghorbani A, Rakhshandeh H. Hypnotic effect of Ocimum basilicum on pentobarbital-induced sleep in mice. Iranian Red Crescent Medical Journal 2016; in press.
  6. Asadpour E, Sadeghnia HR, Ghorbani A, Boroushaki MT. Oxidative stress-mediated cytotoxicity of zirconia nanoparticles on PC12 and N2a cells. Journal of Nanoparticle Research; 2016: 18: 14.
  7. Bihamta M, Hosseini A, Ghorbani A, Boroushaki MT. Protective effect of pomegranate seed oil against H2O2 -induced oxidative stress in cardiomyocytes. Avicenna Journal of Phytomedicine 2016; in press.
  8. Salarinia R. Rakhshandeh H, Oliaee D, Ghasemi SG, Ghorbani A*. Safety evaluation of Phytovagex, a pessary formulation of Nigella sativa, on pregnant rats. Avicenna Journal of Phytomedicine 2016; 6(1): 117-123.
  9. Anaeigoudari A, Hosseini M, Karami R, Vafaee F, Mohammadpour T, Ghorbani A, Sadeghnia HR. The effects of different fractions of Coriandrum sativum on pentylenetetrazole-induced seizures and brain tissues oxidative damage in rats. Avicenna Journal of Phytomedicine 2016; 6(2): 223-235.
  10. Forouzanfar F, Ghorbani A, Hossein M, Rakhshandeh H. Hydroalcoholic extract of needles of Pinus eldarica enhancs pentobarbital-induced sleep; possible involvement of GABAergic system. Avicenna Journal of Phytomedicine 2016; 6(4): 449-457.
  11. Mousavi SH, Naghizade B, Pourgonabadi S, Ghorbani A*. Protective effect of viola tricolor and viola odorata extracts on serum/glucose deprivation-induced neurotoxicity; role of reactive oxygen species. Avicenna Journal of Phytomedicine 2016; 6(4): 434-441.
  12. Abedinzade M, Nasri S, Omodi MJ, Ghasemi E, Ghorbani A. Efficacy of Trigonella foenum-graecum seed in reducing metabolic and inflammatory alterations associated with menopause. Iranian Red Crescent Medical Journal 2015; 17(11): e26685.
  13. Hosseini A, Shafiee-Nick R, Ghorbani A*. Pancreatic beta cell protection/regeneration with phytotherapy. Brazilian Journal of Pharmaceutical Sciences 2015; 51(1): 1-16.
  14. Alinejad B, Shafiee-Nick R, Ghorbani A. Sadeghian H. MC2, a new phosphodiesterase-3 inhibitor with antilipolytic and hypolipidemic effects in normal and diabetic rats. International Journal of Diabetes in Developing Countries 2015; 35(4): 408-417.
  15. Alinejad B, Shafiee-Nick R, Sadeghian H, Ghorbani A. Metabolic effects of newly synthesized phosphodiesterase-3 inhibitor 6-[4-(4-methylpiperidin-1-yl)-4-oxobutoxy]-4-methylquinolin-2(1H)-one on rat adipocytes. DARU Journal of Pharmaceutical Sciences 2015; 23: 19.
  16. Ghorbani A, Asadpour E, Sadeghnia HR. Mechanism of protective effect of lettuce against glucose/serum deprivation-induced neurotoxicity. Nutritional Neuroscience 2015; 18(3): 103-109.
  17. Sadeghnia HR, Taheri E, Ghorbani A. Cigarette smoking behavior and related factors among health professional students in the city of Mashhad (North East of Iran). Iranian Red Crescent Medical Journal 2015; 17(1): e16769.
  18. Ghorbani A, Shafiee-Nick R. Pathological consequences of C-peptide deficiency in insulin-dependent diabetes mellitus. World Journal of Diabetes 2015; 6(1): 145-150.
  19. Hadjzadeh MR, Rajaei Z, Moradi R, Ghorbani A. Effects of hydroalcoholic extract of Watercress (Nasturtium Officinale) leaves on serum glucose and lipid levels in diabetic rats. Indian Journal of Physiology and Pharmacology 2015; 59(2): 223-230.
  20. Javidi M, Zarei M, Omidi S, Ghorbani A, Gharechahi M, Shayani Rad M. Cytotoxicity of a new nano zinc-oxide eugenol sealer on murine fibroblast. Iranian Endodontic Journal 2015; 10(4): 231-235.
  21. Karami R, Hosseini M, Mohammadpour T, Ghorbani A, Sadeghnia HR, Rakhshandeh H, Vafaee F, Esmaeilizadeh M. Effects of hydroalcoholic extract of Coriandrum sativum on oxidative damage in pentylenetetrazole-induced seizures in rats. Iranian Journal of Neurology 2015; 14(2): 59-66.
  22. Hosseini A, GhorbaniA*. Cancer therapy with phytochemicals: evidence from clinical studies. Avicenna Journal of Phytomedicine 2015; 5(2): 84-97.
  23. Kazempor SF, Vafadar Langehbiz S, Hosseini M, Shafei MN, Ghorbani A, Pourganji M. The analgesic effects of different extracts of aerial parts of Coriandrum sativum in mice. International Journal of Biomedical Science 2015; 2(1): 23-28.
  24. Rajaei Z, Hadjzadeh MA, Moradi R, Ghorbani A, Saghebi A. Antihyperglycemic and antihyperlipidemic effects of hydroalcoholic extract of Securigera securidaca seeds in streptozotocin-induced diabetic rats. Advanced Biomedical Research 2015; 4: 33.
  25. Ghorbani A, Salarinia R, Mahdian D,Rakhshandeh H. Effect of hydro-alcoholic extract of Nigella sativa on pregnancy in rat. Iranian Journal of Obstetrics, Gynecology and Infertility 2015; 18 (165): 1-8.
  26. Jamshidi S, Lahouti M, Boroushaki MT, Ganjeali A, Ghorbani A, Bihamta Toosi M. Protective effect of diosgenin against H2O2-induced oxidative stress on H9C2 cells. Journal of Cell and Molecular Research 2015; 7(1): in press.
  27. Ghorbani A, Mogharrabi M, Mohebbati R, Mousavi SM, Hasan zadeh S, Emamian M, Baharloo A, Soukhtanloo M. Effect of long-term administration of Ferula gummosa root extract on serum oxidant-antioxidant status. Iranian Journal of Pharmaceutical Sciences 2014; 10(4): 85-98.
  28. Feizpour A, Boskabady MH, Ghorbani A. Adipose-derived stromal cell therapy affects lung inflammation and tracheal responsiveness in guinea pig model of COPD. PLoS ONE 2014; 9(10): e108974.
  29. Sadeghnia HR, Ghorbani Hesari T, Mortazavian SM, Mousavi SH, Tayarani-Najaran Z, Ghorbani A*. Viola tricolor induces apoptosis in cancer cells and exhibits antiangiogenic activity on chicken chorioallantoic membrane. BioMed Research International 2014; 2014: 625792.
  30. Ghorbani A, Soukhtanloo M, Rajaei Z, Hadjzadeh MAR. Effects of Vaccinium arctostaphylos on lipolysis and adipogenesis in diabetic rats. Research Opinions in Animal & Veterinary Sciences 2014; 4(8): 437-441.
  31. Ghorbani A*, Jalali SA, Varedi M. Isolation of adipose tissue mesenchymal stem cells without tissue destruction: A non-enzymatic method. Tissue & Cell 2014; 46 (1): 54-58.
  32. Ghorbani A, Feizpour A, Hashemzahi M, Gholami L, Hosseini M, Soukhtanloo M, Vafaee Bagheri F, Khodaei E, Roshan M, Boskabady MH. The effect of adipose derived stromal cells on oxidative stress level, lung emphysema and white blood cells of guinea pigs model of chronic obstructive pulmonary disease. DARU Journal of Pharmaceutical Sciences 2014; 22: 26.
    1. Asadpour E, Ghorbani A, Sadeghnia HR. Water-soluble compounds of lettuce inhibit DNA damage and lipid peroxidation induced by glucose/serum deprivation in N2a cells. Acta Poloniae Pharmaceutica Drug Research 2014; 71(3): 409-413.
  33. Ghorbani A, Hadjzadeh MR, Rajaei Z, Zendehbad SB. Effects of fenugreek seeds on adipogenesis and lipolysis in normal and diabetic rat. Pakistan Journal of Biological Sciences 2014; 17 (4): 523-528.
  34. Hosseini A, GhorbaniA, Sadeghnia HR, Rajabian A, Rakhshandeh H. Potentiating effects of Lactuca serriola on pentobarbital-induced sleep. Research Opinions in Animal & Veterinary Sciences 2014; 4(11): 601-607.
  35. Asadpour E, Sadeghnia HR, Ghorbani A, Boroushaki MT. Effect of zirconium dioxide nanoparticles on glutathione peroxidase enzyme activity in N2a and PC12 cell lines. Iranian Journal of Pharmaceutical Research 2014; 13(4): 1141-1148.
  36. Ghorbani A, Moradi Marjaneh R, Rajaei Z, Hadjzadeh MAR. Effects of Securigera securidaca extract on lipolysis and adipogenesis in diabetic rats. Cholestrol 2014; 2014: 582106.
  37. Ghorbani A*. Clinical and experimental studies on polyherbal formulations for diabetes: current status and future prospective. Journal of Integrative Medicine 2014; 12(4): 336-345.
  38. Oliaee D, Boroushaki MT, Oliaee N, Ghorbani A*. Evaluation of cytotoxicity and antifertility effects of Artemisia Kopetdaghensis. Advances in Pharmacological Sciences 2014; 2014: 745760.
  39. Mortazavian SM, Hosainian Z, Ghorbani A*. The effects of hydroalcoholic extract of Dorema aucheri, Stachys pilifera and Trachyspermum copticum on proliferation of rat brain oligodendrocytes. ZUMS Journal 2014; 22 (95): 59-66.
  40. Ghorbani A*, Abedinzade M. Comparison of in vitro and in situ methods for studying lipolysis. ISRN Endocrinology 2013; 2013: 205385.
  41. Ghorbani A, Shafiee-Nick R, Rakhshandeh H, Borji A. Antihyperlipidemic effect of a polyherbal mixture in streptozotocin-induced diabetic rats. Journal of Lipids 2013; 2013: 67579.
  42. Mortazavian SM, Parsaee H, Mousavi SH, Tayarani-Najaran Z, Ghorbani A, Sadeghnia HR. Acetylcholinesterase inhibitors promote angiogenesis in chick chorioallantoic membrane and inhibit apoptosis of endothelial cells. International Journal of Alzheimer's Disease 2013; 2013: 121068.
  43. Alinejad B, Ghorbani A*, Sadeghnia HR. Effects of combinations of curcumin, linalool, rutin, safranal, and thymoquinone on glucose/serum deprivation-induced cell death. Avicenna Journal of Phytomedicine 2013; 3(4): 321-328.
    1. Ghorbani A, Omrani GH, Hadjzadeh MR, Varedi M. Proinsulin C-peptide inhibits lipolysis in diabetic rat adipose tissue through phosphodiestrase-3B enzyme. Hormone and Metabolic Research 2013; 45 (3): 221-225.
    2. Ghorbani A*. Phytotherapy for diabetic dyslipidemia: evidence from clinical trials. Clinical Lipidology 2013; 8 (3): 311-319.
    3. Ghorbani A*. Best herbs for managing diabetes: A review of clinical studies. Brazilian Journal of Pharmaceutical Sciences 2013; 49 (3): 413-422.
  44. Sadeghnia HR, Kamkar M, Assadpour E, Boroushaki MT, Ghorbani A*. Protective effect of safranal, a constituent of Crocus sativus, on quinolinic acid-induced oxidative damage in rat hippocampus. Iranian Journal of Basic Medical Sciences 2013; 16 (1): 73-82.
  45. Forouzanfar F, Goli AA, Assadpour E, Ghorbani A, Sadeghnia HR. Protective effect of Punica granatum L. against serum/glucose deprivation-induced PC12 cells injury. Evidence-based Complementary and Alternative Medicine 2013; 2013; 7167730.
  46. Sadeghnia HR, Yousefsani BS, Rashidfar M, Boroushaki MT, Assadpour E, Ghorbani A. Protective effect of rutin on hexachlorobutadiene-induced nephrotoxicity. Renal Failure 2013; 35 (8): 1151-1155.
  47. GhorbaniA, Rakhshandeh H, Sadeghnia HR. Potentiating effects of Lactuca sativa on pentobarbital-induced sleep. Iranian Journal of Pharmaceutical Research 2013; 12(2): 401-406.
  48. Shafiee-Nick R, Ghorbani A*, Vafaee F, Rakhshandeh H. Chronic administration of a combination of six herbs inhibits the progression of hyperglycemia and decreases serum lipids and aspartate amino transferase activity in diabetic rats. Advances in Pharmacological Sciences 2012; 2012: 789796.
  49. Bostani Khalesi Z, Niknami M, Ghorbani A. Prediction of hypertension by anthropometric parameters in primigravidae. Arya Atherosclerosis 2012; 8: 5 pages.
  50. Hosseini M, Jafarianheris T, Seddighi N, Parvaneh M, Ghorbani A, Sadeghnia HR, Rakhshandeh H. Effects of different extracts of Eugenia caryophyllata on pentylenetetrazole-induced seizures in mice. Journal of Chinese Integrative Medicine 2012; 10(12): 1476-81.
  51. Shafiee-Nick R, Parizadeh SMR, Zokaei N, Ghorbani A. Effects of Ganoderma lucidum hydroalcoholic extract on insulin release in rat-isolated pancreatic islets. Avicenna Journal of Phytomedicine 2012; 2 (4): 206-211.
  52. Ghorbani A, Youssofabad NJ, Rakhshandeh H. Effect of Viola tricolor on pentobarbital-induced sleep in mice. African Journal of Pharmacy and Pharmacology 2012; 6(33): 2503-2509.
  53. Sadeghnia HR, Farahmand SK, Asadpour E, Rakhshandeh H, Ghorbani A*. Neuroprotective effect of Lactuca sativa on glucose/serum deprivation-induced cell death. African Journal of Pharmacy and Pharmacology 2012; 6(33): 2464-2471.
  54. Rakhshandeh H, Sadeghnia HR, Ghorbani A*. Sleep-prolonging effect of Coriandrum sativum hydro-alcoholic extract in mice. Natural Product Research 2012; 26(22): 2095-2098.
  55. Mortazavian SM, Ghorbani A*. Antiproliferative effect of viola tricolor on neuroblastoma cells in vitro. Australian Journal of Medicinal Herbalism 2012; 24 (3): 93-96.
  56. Ghorbani A, Rakhshandeh H, Asadpour E, Sadeghnia HR. Effects of Coriandrum sativum extracts on glucose/serum deprivation-induced neuronal cell death. Avicenna Journal of Phytomedicine 2012; 2 (1): 4-9.61.
  57. Mortazavian SM, Ghorbani A*, Hesari TG. Effect of hydro-alcoholic extract of Viola tricolor and its fractions on proliferation of uterine cervix carcinoma cells. Iranian Journal of Obstetrics, Gynecology and Infertility 2012; 15 (22): 9-16.
  58. Ghorbani A, Omrani GH, Hadjzadeh MR, Varedi M. Effects of rat C-peptide-II on lipolysis and glucose consumption in cultured rat adipose tissue. Experimental and Clinical Endocrinology and Diabetes 2011; 119 (6): 343-347.
  59. Shafiee-Nick R, Parizadeh SMR, Zokaei N, Ghorbani A. Effect of hydro-alcoholic extract of Vaccinium arctostaphylos on insulin release from rat-isolated langerhans islets. Koomesh 2011; 12 (4): 447-452.
  60. Sadeghian A, Ghorbani A, Mohamadi-Nejad A, Rakhshandeh H. Antimicrobial activity of aqueous and methanolic extracts of pomegranate fruit skin. Avicenna Journal of Phytomedicine 2011; 1 (2): 67-73.
  61. Ghorbani A, Varedi M, Hadjzadeh MR, Omrani GH. Type-1 diabetes induces depot-specific alterations in adipocyte diameter and mass of adipose tissues in the rat. Experimental and Clinical Endocrinology and Diabetes 2010; 118 (7): 442-448.
  62. Ghorbani A, Varedi M, Omrani GR. Effects of aging associated weight gain on the cell size and heterogeneity of different fat depots in the rat. Iranian Journal of Endocrinology and Metabolism 2009; 11 (6): 713-720.
  63. Hajzadeh MA, Afshari JT, Ghorbani A*, Shakeri MT. Antiproliferative property of aqueous extract of garlic on human larynx tumour and non-tumour mouse fibroblast cell lines. Australian Journal of Medical Herbalism 2007; 19 (1): 33-37.
  64. Tavakkol-Afshari J, Hajzadeh MR, Ghorbani A*, Parsaie H. Ethanolic extract of Allium sativum has antiproliferative effect on Hep2 and L929 cell lines. Pharmacognosy Magazine 2006; 2 (5): 29-31.
  65. Hajzadeh MR, Rakhshandeh H, Esmaeilzadeh M, Ghorbani A. Analgesic and anti-inflammatory effects of Portolaca Oleracca extract in mice and rat. Koomesh 2004; 5 (3): 113-120.
  66. Fatehi-Hasanabad Z, Ghorbani A, Fatehi M. Study of the cardiovascular effects of Berberis Vulgaris (BV) extract on hypertensive rats and its probable mechanisms. Journal of Sabzevar University of Medical Sciences 2004; 10 (4): 6-12.
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

*: Corresponding Author

 

 

  1. Author: The Most Effective Herbs for Diabetes. Ghorbani A, Rakhshandah H. (Published by Mashhad University of Medical Sciences, 2012).
  2. Translation: Pocket Companion to Guyton and Hall textbook of medical Physiology – John Hall, (Published by Teymoorzadeh, 2013).
  3. Translation: Gastrointestinal Physiology - Leonard R. Johanson, (Published by Mashhad University of Medical Sciences, 2005).
 
 

 

            

  1. Ghorbani A, Ghazvini K. Using a paper-based technique to improve teaching physiology to students of medical sciences. 1st International and 22th Iranian Congress of Physiology and Pharmacology. 7-11 Sep, 2015, Kashan, Iran.
  2. Ghorbani A. Isolation of adipose tissue stem cells with organ culture method. 2nd Annual Congress of Stem Cells and Application. Poster Presentation, 22-23 May, 2014, Mashhad, Iran.
  3. Ghorbani A, Jalali SA, Varedi M. Isolation of human mesenchymal stem cells from very small fat tissue samples; a non-enzymatic method. 21th Iranian Congress of Physiology and Pharmacology. Oral Presentation, 23-27 Aug, 2013, Tabriz, Iran.
  4. Ghorbani A, Asadpour E, Sadeghnia HR. Mechanism of protective effect of lettuce against glucose/serum deprivation-induced neurotoxicity. 21th Iranian Congress of Physiology and Pharmacology. Poster Presentation, 23-27 Aug, 2013, Tabriz, Iran.
  5. Ghorbani A, Rakhshandeh H. Antidiabetic effects of plants that are mentioned in the Quran. Quran & Medicinal Sciences Congress. Poster Presentation, 26-27 Dec, 2012, Mashhad, Iran.
  6. Ghorbani A, Mortazavian SM. Induction of apoptosis in uterine cervix carcinoma cells by ethanolic extract of heartsease. International Multidisciplinary Cancer Congress. Poster Presentation, 4-6 Sep, 2012, Mashhad, Iran.
  7. Ghorbani A, Tavakkol Afshari J, Hadjzadeh MA. A comparison between two garlic extracts for antitumor activity against human laryngeal carcinoma cell. International Multidisciplinary Cancer Congress. Poster Presentation, 4-6 Sep, 2012, Mashhad, Iran.
  8. Ghorbani A. Physiological basis of Islamic and Iranian traditional medicine. The 3rd Conference of Teb-Al-Reza, Oral Presentation, May 2012, Mashhad, Iran.
  9. Ghorbani A. Therapeutic effects of Rosa damasena. The 3rd Conference of Teb-Al-Reza. Poster Presentation, May 2012, Mashhad, Iran.
  10. Ghorbani A, Ranjbar Omrani GH, Hadjzadeh MR, Varedi M. Effects of "connecting peptide" on adipose tissue lipolysis and glucose consumption. 20th Iranian Congress of Physiology and Pharmacology. Oral Presentation, 10-14 Oct, 2011, Hamedan, Iran.
  11. Ghorbani A, Rakhshandeh H, Asadpour E, Sadeghnia HR. Effect of coriander on survival of PC12 cells cultured in glucose/serum-free medium. The 4th International Congress of Biochemistry & Molecular Biology. Oral Presentation, 6-9 Sep, 2011, Mashhad, Iran.
  12. Ghorbani A, Rakhshandeh H, Sadeghnia HR. Hypnotic effect of coriander extract in mice. The 11th International Congress of Iranian Socity of Toxicology. Poster Presentation, 13-15 Sep, 2011, Mashhad, Iran.
  13. Ghorbani A. Review on the use of medicinal plants in aerospace voyage-induced physiological problem. The 10th Conference of Iranian Aerospace Society. Poster Presentation, 29 Feb-2 Mar, 2011, Tehran, Iran.
  14. Ghorbani A. View points of physicians and nurses of health-care centers of Mashhad about medicinal plants and technology development of traditional medicine. The 2nd Conference of Teb-Al-Reza, Poster Presentation, 17 Oct, 2010, Mashhad, Iran.
  15. Ghorbani A, Varedi M, Omrani GH, Hajzadeh MR. Set up of adipose tissue culture in Iran. First congress on research outcoms in Khorassan-Razavi state. Poster Presentation, 18 Dec, 2010, Mashhad, Iran.
  16. Ghorbani A. Epileptogenic properties of some medicinal plants. First International Congress on Epilepsy. Poster Presentation, 10-13 Dec, 2010, Mashhad, Iran.
  17. Ghorbani A. Effect of sport on different aspects of adipose tissue physiology. First Iranian Congress on Sport. Poster Presentation, 23 Dec, 2010, Rasht, Iran.
  18. Ghorbani A, Varedi M, Omrani GH. Effects of aging associated weigh gain on the cell size and heterogeneity of different fat depots in the rat. 2nd Iranian Congress on Prevention and Management of Obesity. Poster Presentation, 27-29 Oct, 2009, Tehran, Iran.
  19. Ghorbani A, Varedi M, Omrani GH. Streptozotocin-induced diabetes alters fat cell size and homogeneity in a depot-specific manner in rat. International Congress on Diabetes and its Complications. Poster Presentation, 19-21 May, 2009, Yazd, Iran.
  20. Ghorbani A, Hajzadeh MR, Sadeghiyan MH. Effects of aqueous and ethanolic extracts of Portolaca Oleracca on rabbit with atherosclerosis disease. 18th Iranian Congress of Physiology & Pharmacology. Poster Presentation, 26-30 Aug, 2007, Mashhad, Iran.
  21. Ghorbani A, Tavakkol-Afshari J, Hajzadeh MR, Parsaie H. Cytotoxic effect of ethanolic extract of Allium sativum on squamous cell carcinoma and non-tumor fibroblast cell line. 17th Iranian Congress of Physiology & Pharmacology. Poster Presentation, 1-4 Oct, 2005, Kerman, Iran.
  22. Ghorbani A, Hajzadeh MR, Omidi AA. Induction of fibroatheroma in rabbit using cholesterol-rich diet. 6th National Congress on Cardiovascular Update. Poster Presentation, 11-14 Jun, 2004, Mashhad, Iran.  
  23. Ghorbani A, Fatehi-Hasanabad Z, Fatehi M. Study of the cardiovascular effects of Berberis Vulgaris extract on hypertensive rats and its probable mechanisms. 16th Iranian Congress of Physiology & Pharmacology. Poster Presentation, 9-13 May, 2003, Tehran, Iran.

 

            

  1.   1.  Ghorbani A, Yoosof-abad NJ, Rakhshandeh H. Sedative-hypnotic effects of Viola tricolor. 2nd International conference on pharmaceutics & novel drug delivery systems. Oral presentation, 20-22 Feb, 2012. San Francisco, USA.
  2.   2.  Ghorbani A, Omrani GR, Hadjzadeh MR, Varedi M. Antilipolytic effect of proinsulin C-peptide in diabetic rat. International conference and exhibition on metabolism & systems biology. Oral presentation, 20-22 Feb, 2012, San Francisco, USA.
  3.   3.  Ghorbani A, Ghorbani T, Mousavi SH, Tayarani-Najaran Z, Mortazavian SM, Sadeghnia, HR, Rakhshandeh H. Ethyl acetate fraction of pansy (Viola tricolor) induces cytotoxic effect against N2a cancer cell line. The second annual meeting of Middle-Eastern Association for Cancer Research. Oral presentation, 27-29 Nov, 2012. Sfax, Tunisia.
  4.   4.  Ghorbani A, Shafiee-Nick R, Vafaee Bagheri F, Rakhshandeh H. Development of polyherbal mixtures for management of diabetic dyslipidemia. 2013 International conference on diabetes and metabolism & 5th scientific meeting of the Asian Association for the Study of Diabetes. Poster presentation, 6-9 Nov, 2013. Seoul, South Korea.
  5.   5.  Ghorbani A, Hadjzadeh MR, Rajaei Z, Zendehbad SB. Effects of Trigonella foenum-graecum on lipolysis and adipogenesis. 2013 International conference on diabetes and metabolism & 5th scientific meeting of the Asian Association for the Study of Diabetes. Oral presentation, 6-9 Nov, 2013. Seoul, South Korea.
  6.   6.  Ghorbani A, Rakhshandeh H, Sadeghnia HR, Hosseini A. Management of mood and anxiety with medicinal plants. 14th International Forum on Mood and Anxiety Disorders. Poster presentation, 10-12 Dec, 2014. Vienna, Austria.
  7.   7.  Ghorbani A, Sadeghnia HR, Arjmand F. Neuroprotective effect of Boswellia serrata and its active constituent acetyl 11-keto beta boswellic acid. 14th International Forum on Mood and Anxiety Disorders. Poster presentation, 10-12 Dec, 2014. Vienna, Austria.
  8.   8.  Ghorbani A, Hosseini A, Mojarad M, Hatami A, Hoseyni SJ. Effect of diabetes on proliferation and differentiation abilities of mesenchymal stem cells isolated from subcutaneous and visceral adipose tissue. 7th Asian Association for the Study of Diabetes (AASD) Scientific Meeting and Annual Scientific Meeting of the Hong Kong Society of Endocrinology, Metabolism and Reproduction. Poster presentation, 20-21 Nov, 2015. Hong Kong.
  9.   9.  Ghorbani A, Naderi-Meshkin H. The endocrine regulation of stem and progenitor cells. 7th Asian Association for the Study of Diabetes (AASD) Scientific Meeting and Annual Scientific Meeting of the Hong Kong Society of Endocrinology, Metabolism and Reproduction. Poster presentation, 20-21 Nov, 2015. Hong Kong.